Poster Session A   |   11:45am Expo - Hall A & C   |   Poster ID #117

Identification of Image-based Markers of Human Oral Squamous Cell Carcinoma (OSCC) Using Multiphoton Microscopy

Academic Research
Prevention, Early Detection, Implementation, and Dissemination
FDA Status:
Not Applicable
CPRIT Grant:
Cancer Site(s):
Head and Neck, Esophagus, HPV-related, Tobacco-related
Paula Villarreal
The University of Texas Medical Branch at Galveston
Rahul Pal
Harvard T.H. Chan School of Public Health
Suimin Qiu
The University of Texas Medical Branch at Galveston
Orly Coblens
The University of Texas Medical Branch at Galveston
Gracie Vargas
The University of Texas Medical Branch at Galveston


Depth-resolved label-free optical imaging by the method of multiphoton autofluorescence microscopy (MPAM) may offer new ways to examine cellular and extracellular atypia associated with epithelial squamous cell carcinoma (SCC). MPAM was evaluated for its ability to identify cellular and microstructural atypia in head and neck tissues from resected discarded tumor tissue. 


Three-dimensional image volumes were obtained from tissues from the floor of the mouth, tongue, and larynx, and were then processed for histology. MPAM micrographs were evaluated for qualitative metrics of cell atypia and quantitative measures associated with nuclear pleomorphism. Statistical analyses correlated MPAM endpoints with histological grade from each imaged site.


Cellular overcrowding, discohesion, anisonucleosis, and multinucleated cells, as observed through MPAM, were found to be statistically associated with dysplasia and SCC grading, but not in histologically benign regions. A quantitative measure of the coefficient of variance in nuclear size in SCC and dysplasia was statistically elevated above histologically benign regions. MPAM also allowed for the identification of cellular heterogeneity across transitional areas and other features, such as inflammatory infiltrates. 


In the future, MPAM could be evaluated for the non-invasive detection of neoplasia, possibly as an adjunct to traditional conventional examination and biopsy.